The US Open Cup's Round of 16 will feature 13 teams from Major League Soccer, and their matchups were revealed in Thursday evening's draw.

Three lower-division teams are left standing, and two will host when quarterfinal berths are determined on May 25.

Here's what awaits in the four regions, with all-MLS matchups including El Trafico and the Florida Derby.

Northeast Region

Southeast Region

Central Region

West Region

Who will win the U.S. Open Cup?